Geoduck Rna

Exploring RNA-Seq data from Illumina effort

Here is what Sam sent

The 12 samples will be used for RNAseq for genome annotation – numbers indicate desired sequencing priority.

Juvenile and larval samples were from Hollie Putnam (see links below for more info).

Other tissue was from a single, adult geoduck, collected by Brent & Steven on 20150811.

Juvenile OA exposure (super low)  (EPI_115, EPI_116)
Juvenile ambient exposure (ambient treatment) (EPI_123, EPI_124)
Larvae day 0 (EPI_74, EPI_75)
Larvae day 5 (EPI_99)
Crystalline style
Byssus gland
Labial palps
Juvenile OA exposure – low treatment (EPI_107, EPI_108)



Larval Files

These seem to be same library (PE ???) split over two lanes.

NovaSeq run for all combined. The MP libraries have a 800 bp insert and the RNA Seq libraries have a 300bp insert so they were loaded in a 10:1 MP:RNA into the NovaSeq.

Will attempt Trinity with larval samples- maybe Hyak… Guidance…

Written on August 3, 2017