PRJNA593309 (heat) RefSeq functional annotations

Descriptions and gene biotypes come directly from GCF_963853765.1 / RS_2024_06. This is annotation and ranking, not ontology or pathway enrichment.

Heat Higher Fdr Lt 0.05

Genes: 30; RefSeq-characterized descriptions: 18 (60.0%).

  • LOC105342383 (NCBI:GeneID:105342383): rhamnose-binding lectin (log2FC=2.99; adjusted p=6.81e-07)
  • LOC117684474 (NCBI:GeneID:117684474): NFX1-type zinc finger-containing protein 1 (log2FC=2.29; adjusted p=3.66e-05)
  • LOC117680356 (NCBI:GeneID:117680356): mucin-2-like (log2FC=4.03; adjusted p=0.000556)
  • LOC117687305 (NCBI:GeneID:117687305): talin-1-like (log2FC=2.07; adjusted p=0.00325)
  • LOC105340828 (NCBI:GeneID:105340828): heme-binding protein 2 (log2FC=2.23; adjusted p=0.00374)
  • LOC105322994 (NCBI:GeneID:105322994): E3 SUMO-protein ligase EGR2 (log2FC=1.71; adjusted p=0.0103)
  • LOC105340534 (NCBI:GeneID:105340534): deoxynucleoside triphosphate triphosphohydrolase SAMHD1 (log2FC=2.73; adjusted p=0.0108)
  • LOC136276213 (NCBI:GeneID:136276213): beta-hexosaminidase-like (log2FC=1.86; adjusted p=0.0139)
  • LOC105338118 (NCBI:GeneID:105338118): ski oncogene (log2FC=1.20; adjusted p=0.021)
  • LOC105319208 (NCBI:GeneID:105319208): spliceosome RNA helicase DDX39B (log2FC=2.22; adjusted p=0.0304)
  • LOC105339781 (NCBI:GeneID:105339781): lactadherin (log2FC=1.31; adjusted p=0.033)
  • LOC105347396 (NCBI:GeneID:105347396): sodium/hydrogen exchanger 9B2 (log2FC=2.60; adjusted p=0.0346)
  • LOC117692972 (NCBI:GeneID:117692972): sal-like protein 1 (log2FC=1.58; adjusted p=0.0359)
  • LOC105321656 (NCBI:GeneID:105321656): multidrug resistance-associated protein 1 (log2FC=2.17; adjusted p=0.0421)
  • LOC105319458 (NCBI:GeneID:105319458): dystroglycan 1 (log2FC=1.46; adjusted p=0.046)

Heat Lower Fdr Lt 0.05

Genes: 168; RefSeq-characterized descriptions: 81 (48.2%).

  • LOC105318596 (NCBI:GeneID:105318596): NPC intracellular cholesterol transporter 2 (log2FC=-3.53; adjusted p=2.98e-12)
  • LOC105343649 (NCBI:GeneID:105343649): cerebellin-1 (log2FC=-5.05; adjusted p=1.22e-09)
  • LOC105327353 (NCBI:GeneID:105327353): natterin-4-like (log2FC=-3.66; adjusted p=1.38e-09)
  • LOC105340688 (NCBI:GeneID:105340688): NPC intracellular cholesterol transporter 2 (log2FC=-6.80; adjusted p=8.17e-09)
  • LOC117687426 (NCBI:GeneID:117687426): annexin A7-like (log2FC=-2.31; adjusted p=2.4e-08)
  • LOC105330356 (NCBI:GeneID:105330356): ETS-related transcription factor Elf-5 (log2FC=-3.10; adjusted p=4.5e-08)
  • LOC105337817 (NCBI:GeneID:105337817): A disintegrin and metalloproteinase with thrombospondin motifs adt-1 (log2FC=-2.71; adjusted p=2.59e-07)
  • LOC105346137 (NCBI:GeneID:105346137): von Willebrand factor D and EGF domain-containing protein (log2FC=-4.96; adjusted p=7.17e-06)
  • LOC136271313 (NCBI:GeneID:136271313): putative uncharacterized protein DDB_G0282133 (log2FC=-8.70; adjusted p=1.75e-05)
  • LOC136276340 (NCBI:GeneID:136276340): perlucin-like (log2FC=-5.22; adjusted p=2.36e-05)
  • LOC136269603 (NCBI:GeneID:136269603): allene oxide synthase-lipoxygenase protein-like (log2FC=-9.38; adjusted p=3.66e-05)
  • LOC105347917 (NCBI:GeneID:105347917): macrophage-expressed gene 1 protein (log2FC=-2.72; adjusted p=5.63e-05)
  • LOC105337888 (NCBI:GeneID:105337888): protein shisa-5 (log2FC=-3.63; adjusted p=6.02e-05)
  • LOC117688787 (NCBI:GeneID:117688787): spidroin-1-like (log2FC=-3.30; adjusted p=7.86e-05)
  • LOC105327731 (NCBI:GeneID:105327731): serine protease inhibitor Cvsi-1 (log2FC=-2.58; adjusted p=0.000119)

Gene-biotype over-representation

One-sided hypergeometric tests compare each significant direction with all genes having DESeq2 results; Benjamini-Hochberg adjustment covers both directions and all biotypes.

  • heat higher fdr lt 0.05: lncRNA (5 observed, 1.6 expected; fold=3.17; adjusted p=0.0472)
  • heat lower fdr lt 0.05: lncRNA (26 observed, 8.8 expected; fold=2.95; adjusted p=3.39e-06)
  • heat lower fdr lt 0.05: misc_RNA (7 observed, 1.1 expected; fold=6.10; adjusted p=0.000503)
  • heat lower fdr lt 0.05: rRNA (5 observed, 0.2 expected; fold=29.65; adjusted p=3.39e-06)

Many oyster loci retain automated or uncharacterized RefSeq labels. Functional claims should be based on formal ontology/pathway enrichment or orthology-aware analysis rather than names alone.