PRJNA694496 functional enrichment

Over-representation analysis used g:Profiler with the version-matched Magallana gigas GCF_963853765.1 organism build. Selected-higher and selected-lower genes were tested separately against genes with nonmissing DESeq2 p-values. g:SCS-adjusted p < 0.05 defines significance.

All direction-specific RefSeq symbols mapped without failure or ambiguity. This organism build supplied GO Biological Process, Molecular Function, and Cellular Component annotations; requested KEGG, Reactome, and WikiPathways sources were unavailable.

Selected Higher

Significant terms: 35.

  • GO:MF GO:0003723: RNA binding (adjusted p=1.89e-11; overlap=171/4353)
  • GO:BP GO:0006396: RNA processing (adjusted p=5.92e-11; overlap=127/4353)
  • GO:CC GO:1990904: ribonucleoprotein complex (adjusted p=1.61e-08; overlap=92/4353)
  • GO:BP GO:0016070: RNA metabolic process (adjusted p=7.06e-06; overlap=300/4353)
  • GO:MF GO:0003676: nucleic acid binding (adjusted p=1.15e-05; overlap=405/4353)
  • GO:BP GO:0000377: RNA splicing, via transesterification reactions with bulged adenosine as nucleophile (adjusted p=2.05e-05; overlap=43/4353)
  • GO:BP GO:0000398: mRNA splicing, via spliceosome (adjusted p=2.05e-05; overlap=43/4353)
  • GO:BP GO:0006397: mRNA processing (adjusted p=2.5e-05; overlap=59/4353)
  • GO:BP GO:0000375: RNA splicing, via transesterification reactions (adjusted p=3.07e-05; overlap=43/4353)
  • GO:BP GO:0016071: mRNA metabolic process (adjusted p=3.08e-05; overlap=68/4353)
  • GO:BP GO:0010467: gene expression (adjusted p=3.1e-05; overlap=375/4353)
  • GO:BP GO:0032774: RNA biosynthetic process (adjusted p=8.39e-05; overlap=272/4353)
  • GO:BP GO:0008380: RNA splicing (adjusted p=0.000101; overlap=51/4353)
  • GO:BP GO:0090304: nucleic acid metabolic process (adjusted p=0.000106; overlap=390/4353)
  • GO:BP GO:0009059: macromolecule biosynthetic process (adjusted p=0.000198; overlap=382/4353)

Selected Lower

Significant terms: 108.

  • GO:MF GO:0003824: catalytic activity (adjusted p=8.12e-27; overlap=1167/4449)
  • GO:BP GO:0044281: small molecule metabolic process (adjusted p=4.46e-13; overlap=166/4449)
  • GO:CC GO:0005856: cytoskeleton (adjusted p=8.74e-12; overlap=144/4449)
  • GO:BP GO:0006793: phosphorus metabolic process (adjusted p=8.91e-12; overlap=257/4449)
  • GO:BP GO:0006796: phosphate-containing compound metabolic process (adjusted p=9.88e-12; overlap=256/4449)
  • GO:MF GO:0016740: transferase activity (adjusted p=1.65e-11; overlap=416/4449)
  • GO:MF GO:0016491: oxidoreductase activity (adjusted p=5.63e-11; overlap=212/4449)
  • GO:MF GO:1901363: heterocyclic compound binding (adjusted p=3.61e-10; overlap=444/4449)
  • GO:MF GO:0140096: catalytic activity, acting on a protein (adjusted p=6.28e-10; overlap=392/4449)
  • GO:BP GO:0006082: organic acid metabolic process (adjusted p=1.35e-09; overlap=82/4449)
  • GO:BP GO:0043436: oxoacid metabolic process (adjusted p=1.35e-09; overlap=81/4449)
  • GO:BP GO:0019752: carboxylic acid metabolic process (adjusted p=1.95e-09; overlap=79/4449)
  • GO:CC GO:0005737: cytoplasm (adjusted p=5.06e-09; overlap=482/4449)
  • GO:CC GO:0005575: cellular_component (adjusted p=5.37e-09; overlap=1312/4449)
  • GO:MF GO:0043168: anion binding (adjusted p=7.22e-09; overlap=431/4449)

Terms are descriptive population-selection associations. Related ontology terms are not statistically independent, and enrichment does not establish causal thermotolerance mechanisms.