PRJNA913164 (heat) RefSeq functional annotations

Descriptions and gene biotypes come directly from GCF_963853765.1 / RS_2024_06. This is annotation and ranking, not ontology or pathway enrichment.

Heat Higher Fdr Lt 0.05

Genes: 2,216; RefSeq-characterized descriptions: 1,576 (71.1%).

  • LOC105342400 (NCBI:GeneID:105342400): serine/arginine-rich splicing factor 4 (log2FC=1.68; adjusted p=2.32e-117)
  • LOC105341070 (NCBI:GeneID:105341070): DNA topoisomerase I, mitochondrial (log2FC=1.46; adjusted p=6.14e-108)
  • Crt (NCBI:GeneID:105336966): calreticulin (log2FC=1.89; adjusted p=2.06e-95)
  • LOC117680708 (NCBI:GeneID:117680708): serine/arginine-rich splicing factor 1 (log2FC=1.79; adjusted p=6.89e-73)
  • LOC105342698 (NCBI:GeneID:105342698): probable splicing factor, arginine/serine-rich 6 (log2FC=1.42; adjusted p=9.3e-54)
  • LOC105341388 (NCBI:GeneID:105341388): protein disulfide-isomerase A6 (log2FC=1.94; adjusted p=2.1e-51)
  • LOC105348083 (NCBI:GeneID:105348083): MAP kinase-interacting serine/threonine-protein kinase 1 (log2FC=1.06; adjusted p=5.67e-50)
  • LOC105340370 (NCBI:GeneID:105340370): 78 kDa glucose-regulated protein (log2FC=1.81; adjusted p=3.76e-49)
  • LOC105330746 (NCBI:GeneID:105330746): nuclear factor of activated T-cells 5 (log2FC=0.76; adjusted p=2.26e-44)
  • LOC105326121 (NCBI:GeneID:105326121): cysteine-rich with EGF-like domain protein 2 (log2FC=2.00; adjusted p=8.01e-44)
  • LOC105324846 (NCBI:GeneID:105324846): stalled ribosome sensor GCN1 (log2FC=1.07; adjusted p=6.39e-43)
  • LOC105334027 (NCBI:GeneID:105334027): Krueppel-like factor 8 (log2FC=0.69; adjusted p=2.17e-38)
  • LOC105337016 (NCBI:GeneID:105337016): protein c-Fos (log2FC=2.60; adjusted p=6.93e-36)
  • LOC105326739 (NCBI:GeneID:105326739): RNA-binding protein lark (log2FC=0.98; adjusted p=1.83e-35)
  • LOC105344299 (NCBI:GeneID:105344299): LRP chaperone MESD (log2FC=1.00; adjusted p=6.86e-29)

Heat Lower Fdr Lt 0.05

Genes: 2,178; RefSeq-characterized descriptions: 1,567 (71.9%).

  • LOC105329085 (NCBI:GeneID:105329085): high mobility group-T protein (log2FC=-2.21; adjusted p=1.32e-133)
  • LOC105323271 (NCBI:GeneID:105323271): zinc finger CCHC domain-containing protein 17 (log2FC=-1.55; adjusted p=3.27e-78)
  • LOC105344085 (NCBI:GeneID:105344085): nucleolin (log2FC=-2.04; adjusted p=6.53e-72)
  • LOC105347167 (NCBI:GeneID:105347167): heterogeneous nuclear ribonucleoprotein D-like (log2FC=-1.11; adjusted p=3.52e-54)
  • Hmgb1 (NCBI:GeneID:105348423): high mobility group box 1 (log2FC=-1.73; adjusted p=9.56e-43)
  • LOC105338803 (NCBI:GeneID:105338803): putative RNA-binding protein Luc7-like 2 (log2FC=-0.67; adjusted p=5e-40)
  • LOC109618881 (NCBI:GeneID:109618881): tubulin alpha-1A chain (log2FC=-1.72; adjusted p=1.89e-30)
  • LOC105317162 (NCBI:GeneID:105317162): adenosylhomocysteinase A (log2FC=-1.58; adjusted p=2.94e-29)
  • LOC105345801 (NCBI:GeneID:105345801): CD109 antigen (log2FC=-1.37; adjusted p=2.66e-28)
  • LOC105345927 (NCBI:GeneID:105345927): zinc finger protein ubi-d4 (log2FC=-0.79; adjusted p=8.86e-28)
  • LOC105339476 (NCBI:GeneID:105339476): influenza virus NS1A-binding protein (log2FC=-1.13; adjusted p=2.2e-26)
  • LOC105345357 (NCBI:GeneID:105345357): 5’-3’ exoribonuclease 2 (log2FC=-0.83; adjusted p=1.17e-25)
  • LOC105335265 (NCBI:GeneID:105335265): lys-63-specific deubiquitinase BRCC36 (log2FC=-1.03; adjusted p=8.75e-24)
  • LOC105347281 (NCBI:GeneID:105347281): eukaryotic translation initiation factor 3 subunit H (log2FC=-0.98; adjusted p=5.15e-22)
  • LOC105321121 (NCBI:GeneID:105321121): annexin A7 (log2FC=-0.77; adjusted p=5.74e-22)

Gene-biotype over-representation

One-sided hypergeometric tests compare each significant direction with all genes having DESeq2 results; Benjamini-Hochberg adjustment covers both directions and all biotypes.

  • heat higher fdr lt 0.05: protein_coding (2054 observed, 1998.3 expected; fold=1.03; adjusted p=4.66e-05)
  • heat lower fdr lt 0.05: protein_coding (2042 observed, 1964.0 expected; fold=1.04; adjusted p=2.47e-09)

Many oyster loci retain automated or uncharacterized RefSeq labels. Functional claims should be based on formal ontology/pathway enrichment or orthology-aware analysis rather than names alone.