PRJNA913164 (heat_emersion) RefSeq functional annotations

Descriptions and gene biotypes come directly from GCF_963853765.1 / RS_2024_06. This is annotation and ranking, not ontology or pathway enrichment.

Heat Emersion Higher Fdr Lt 0.05

Genes: 1,655; RefSeq-characterized descriptions: 1,179 (71.2%).

  • LOC105342400 (NCBI:GeneID:105342400): serine/arginine-rich splicing factor 4 (log2FC=1.59; adjusted p=2.43e-102)
  • Crt (NCBI:GeneID:105336966): calreticulin (log2FC=1.64; adjusted p=2.96e-59)
  • LOC117680708 (NCBI:GeneID:117680708): serine/arginine-rich splicing factor 1 (log2FC=1.68; adjusted p=3.49e-52)
  • LOC105334510 (NCBI:GeneID:105334510): heat shock protein 68-like (log2FC=8.48; adjusted p=1.01e-43)
  • LOC117680673 (NCBI:GeneID:117680673): heat shock protein 68 (log2FC=8.47; adjusted p=2.12e-43)
  • LOC105342698 (NCBI:GeneID:105342698): probable splicing factor, arginine/serine-rich 6 (log2FC=1.47; adjusted p=4.84e-41)
  • LOC105348305 (NCBI:GeneID:105348305): heat shock protein 68-like (log2FC=7.61; adjusted p=8.9e-41)
  • LOC105322484 (NCBI:GeneID:105322484): heat shock protein 68-like (log2FC=5.52; adjusted p=1.42e-40)
  • LOC105343420 (NCBI:GeneID:105343420): endothelial differentiation-related factor 1 (log2FC=0.67; adjusted p=1.22e-38)
  • LOC105348304 (NCBI:GeneID:105348304): heat shock protein 68 (log2FC=8.11; adjusted p=5.87e-37)
  • LOC105341070 (NCBI:GeneID:105341070): DNA topoisomerase I, mitochondrial (log2FC=0.86; adjusted p=2.87e-33)
  • LOC105325416 (NCBI:GeneID:105325416): inactive rhomboid protein 2 (log2FC=3.21; adjusted p=5.6e-32)
  • LOC105333434 (NCBI:GeneID:105333434): heterogeneous nuclear ribonucleoprotein A3 (log2FC=0.64; adjusted p=6.33e-30)
  • LOC105322320 (NCBI:GeneID:105322320): protein disulfide-isomerase A4 (log2FC=1.40; adjusted p=5.11e-28)
  • LOC105326739 (NCBI:GeneID:105326739): RNA-binding protein lark (log2FC=0.83; adjusted p=8.86e-26)

Heat Emersion Lower Fdr Lt 0.05

Genes: 1,581; RefSeq-characterized descriptions: 1,163 (73.6%).

  • LOC105329085 (NCBI:GeneID:105329085): high mobility group-T protein (log2FC=-1.63; adjusted p=3.78e-68)
  • Hmgb1 (NCBI:GeneID:105348423): high mobility group box 1 (log2FC=-1.71; adjusted p=5.11e-60)
  • LOC105323271 (NCBI:GeneID:105323271): zinc finger CCHC domain-containing protein 17 (log2FC=-1.32; adjusted p=5.9e-36)
  • LOC105344085 (NCBI:GeneID:105344085): nucleolin (log2FC=-1.77; adjusted p=1.28e-35)
  • LOC105347052 (NCBI:GeneID:105347052): ribosomal L1 domain-containing protein 1 (log2FC=-1.13; adjusted p=2.2e-32)
  • LOC105345357 (NCBI:GeneID:105345357): 5’-3’ exoribonuclease 2 (log2FC=-0.99; adjusted p=3.6e-32)
  • LOC105348951 (NCBI:GeneID:105348951): protein mono-ADP-ribosyltransferase PARP12 (log2FC=-0.62; adjusted p=2.06e-26)
  • LOC105347281 (NCBI:GeneID:105347281): eukaryotic translation initiation factor 3 subunit H (log2FC=-0.76; adjusted p=5.04e-23)
  • LOC105323703 (NCBI:GeneID:105323703): enolase 4 (log2FC=-0.65; adjusted p=2.96e-21)
  • LOC105339136 (NCBI:GeneID:105339136): transcriptional regulator ATRX homolog (log2FC=-0.87; adjusted p=9.09e-19)
  • LOC105347167 (NCBI:GeneID:105347167): heterogeneous nuclear ribonucleoprotein D-like (log2FC=-0.60; adjusted p=1.5e-16)
  • LOC105320480 (NCBI:GeneID:105320480): BUD13 homolog (log2FC=-0.52; adjusted p=4.78e-16)
  • LOC105325240 (NCBI:GeneID:105325240): sodium- and chloride-dependent taurine transporter-like (log2FC=-0.66; adjusted p=4.78e-16)
  • LOC105321121 (NCBI:GeneID:105321121): annexin A7 (log2FC=-0.65; adjusted p=5.51e-16)
  • LOC105341443 (NCBI:GeneID:105341443): DNA-binding protein HEXBP (log2FC=-0.47; adjusted p=1.38e-15)

Gene-biotype over-representation

One-sided hypergeometric tests compare each significant direction with all genes having DESeq2 results; Benjamini-Hochberg adjustment covers both directions and all biotypes.

  • heat emersion higher fdr lt 0.05: snRNA (9 observed, 0.9 expected; fold=9.68; adjusted p=3.25e-08)
  • heat emersion higher fdr lt 0.05: snoRNA (2 observed, 0.2 expected; fold=10.76; adjusted p=0.0403)
  • heat emersion lower fdr lt 0.05: protein_coding (1493 observed, 1426.8 expected; fold=1.05; adjusted p=3.03e-09)

Many oyster loci retain automated or uncharacterized RefSeq labels. Functional claims should be based on formal ontology/pathway enrichment or orthology-aware analysis rather than names alone.